|Application ||WB, IHC-P, IF, E|
|Dilution||IHC-P (5 µg/ml), WB (1-2 µg/ml),|
|Other Names||Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y, PIGY|
|Target/Specificity||The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kD in SDS-PAGE.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||PIGY Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the GPI-GlcNAc transferase (GPI-GnT) complex in the endoplasmic reticulum, a complex that catalyzes transfer of GlcNAc from UDP-GlcNAc to an acceptor phosphatidylinositol, the first step in the production of GPI- anchors for cell surface proteins. May act by regulating the catalytic subunit PIGA.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Component of the GPI-GlcNAc transferase (GPI-GnT) complex in the endoplasmic reticulum, a complex that catalyzes transfer of GlcNAc from UDP-GlcNAc to an acceptor phosphatidylinositol, the first step in the production of GPI- anchors for cell surface proteins. May act by regulating the catalytic subunit PIGA.
Murakami Y.,et al.Mol. Biol. Cell 16:5236-5246(2005).
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