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POLG Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | P54098 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 140kDa |
| Dilution | ICC (1:100-1:200), IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:1000-1:10000) , |
| Gene ID | 5428 |
|---|---|
| Other Names | DNA polymerase subunit gamma-1, 2.7.7.7, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA |
| Target/Specificity | Human DNA polymerase gamma. Predicted cross-reactivity based on amino acid sequence homology: mouse (91%), rat (91%). |
| Reconstitution & Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Precautions | POLG Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLG {ECO:0000303|PubMed:10827171, ECO:0000312|HGNC:HGNC:9179} |
|---|---|
| Function | Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates (PubMed:9558343, PubMed:11477093, PubMed:19837034, PubMed:11897778, PubMed:15917273). Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double- stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post- insertion site. After completing replication of a mtDNA strand, mediates 3' -> 5' exonucleolytic degradation at the nick to enable proper ligation (PubMed:9558343, PubMed:11477093, PubMed:15167897, PubMed:26095671, PubMed:19837034, PubMed:11897778, PubMed:15917273). Highly accurate due to high nucleotide selectivity and 3' -> 5' exonucleolytic proofreading. Proficiently corrects base substitutions, single-base additions and deletions in non-repetitive sequences and short repeats, but displays lower proofreading activity when replicating longer homopolymeric stretches. Exerts exonuclease activity toward single-stranded DNA and double-stranded DNA containing 3'- terminal mispairs. When a misincorporation occurs, transitions from replication to a pro-nucleolytic editing mode and removes the missincorporated nucleoside in the exonuclease active site. Proceeds via an SN2 nucleolytic mechanism in which Asp-198 catalyzes phosphodiester bond hydrolysis and Glu-200 stabilizes the leaving group. As a result the primer strand becomes one nucleotide shorter and is positioned in the post-insertion site, ready to resume DNA synthesis (PubMed:10827171, PubMed:11477094, PubMed:11504725, PubMed:37202477). Exerts 5'-deoxyribose phosphate (dRP) lyase activity and mediates repair-associated mtDNA synthesis (gap filling) in base-excision repair pathway. Catalyzes the release of the 5'-terminal 2-deoxyribose-5- phosphate sugar moiety from incised apurinic/apyrimidinic (AP) sites to produce a substrate for DNA ligase. The dRP lyase reaction does not require divalent metal ions and likely proceeds via a Schiff base intermediate in a beta-elimination reaction mechanism (PubMed:9770471). |
| Cellular Location | Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid |
| Volume | 50 µl |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA.
References
Ropp P.A.,et al.Genomics 36:449-458(1996).
Lecrenier N.L.,et al.Gene 185:147-152(1997).
Watanabe T.K.,et al.Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
Bogenhagen D.F.,et al.J. Biol. Chem. 283:3665-3675(2008).
Van Goethem G.,et al.Nat. Genet. 28:211-212(2001).
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$ 467.50
Cat# ALS13141
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