KARS Antibody (clone 8G12C1)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | Q15046 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 8G12C1 |
Calculated MW | 68kDa |
Dilution | ELISA (1:10000), IHC-P (1:200), WB (1:500-1:2000) |
Gene ID | 3735 |
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Other Names | Lysine--tRNA ligase, 6.1.1.6, Lysyl-tRNA synthetase, LysRS, KARS, KIAA0070 |
Target/Specificity | Human KARS |
Reconstitution & Storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
Precautions | KARS Antibody (clone 8G12C1) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | KARS1 (HGNC:6215) |
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Synonyms | KARS, KIAA0070 |
Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:9278442, PubMed:18029264, PubMed:18272479). When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages (PubMed:15851690). Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity (PubMed:5338216, PubMed:14975237, PubMed:19524539, PubMed:23159739). |
Cellular Location | [Isoform Cytoplasmic]: Cytoplasm, cytosol. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Secreted Note=Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739) |
Volume | 50 µl |
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Background
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
References
Shiba K.,et al.J. Biol. Chem. 272:22809-22816(1997).
Tolkunova E.,et al.J. Biol. Chem. 275:35063-35069(2000).
Nomura N.,et al.DNA Res. 1:223-229(1994).
Martin J.,et al.Nature 432:988-994(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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