|Application ||WB, IHC-P, E|
|Dilution||ELISA (1:10000), IHC-P (1:200), WB (1:500-1:2000)|
|Other Names||Apolipoprotein O, Protein FAM121B, APOO, FAM121B|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||APOO / Apolipoprotein O Antibody (clone 2F1) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Plays a crucial role in crista junction formation and mitochondrial function (PubMed:25764979). Can promote cardiac lipotoxicity by enhancing mitochondrial respiration and fatty acid metabolism in cardiac myoblasts (PubMed:24743151). Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL (PubMed:16956892).|
|Cellular Location||Mitochondrion inner membrane; Single-pass membrane protein. Secreted. Mitochondrion. Golgi apparatus membrane. Endoplasmic reticulum membrane. Note=Exists in three distinct forms: a glycosylated and secreted form, an ER/Golgi-resident form and a non-glycosylated mitochondrial form|
|Tissue Location||Expressed in all tissues examined. Up- regulated in diabetic heart.|
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Provided below are standard protocols that you may find useful for product applications.
Promotes cholesterol efflux from macrophage cells. Detected in HDL, LDL and VLDL. Secreted by a microsomal triglyceride transfer protein (MTTP)-dependent mechanism, probably as a VLDL-associated protein that is subsequently transferred to HDL. May be involved in myocardium-protective mechanisms against lipid accumulation.
Mao Y.M.,et al.Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lamant M.,et al.J. Biol. Chem. 281:36289-36302(2006).
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