|Application ||WB, IHC-P, E, Flo|
|Dilution||ELISA (1:10000), Flo (1:200-1:400), IHC-P (1:200), WB (1:500-1:2000) ,|
|Other Names||Protein LYRIC, 3D3/LYRIC, Astrocyte elevated gene-1 protein, AEG-1, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, MTDH, AEG1, LYRIC|
|Reconstitution & Storage||Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.|
|Precautions||MTDH / Metadherin Antibody (clone 2F11C3) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF- kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Cell junction, tight junction. Nucleus, nucleolus. Cytoplasm, perinuclear region. Note=In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.|
|Tissue Location||Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme|
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Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF- kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
Sutherland H.G.E.,et al.Exp. Cell Res. 294:94-105(2004).
Britt D.E.,et al.Exp. Cell Res. 300:134-148(2004).
Kang D.-C.,et al.Gene 353:8-15(2005).
Liang Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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