|Application ||WB, IHC-P, IF, ICC, Flo|
|Dilution||ICC (1:100-1:200), IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000) ,|
|Other Names||POU domain, class 5, transcription factor 1, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, POU5F1, OCT3, OCT4, OTF3|
|Target/Specificity||Human OCT3/4. Predicted cross-reactivity based on amino acid sequence homology: rabbit (85%), bovine (85%), pig (88%), monkey (97%).|
|Reconstitution & Storage||Aliquot and store at -20°C. Minimize freezing and thawing.|
|Precautions||POU5F1 / OCT4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||OCT3, OCT4, OTF3|
|Function||Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.|
|Cellular Location||Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern|
|Tissue Location||Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.|
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Provided below are standard protocols that you may find useful for product applications.
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Takeda J.,et al.Nucleic Acids Res. 20:4613-4620(1992).
Stuart P.E.,et al.Tissue Antigens 76:387-397(2010).
Shiina T.,et al.Genetics 173:1555-1570(2006).
Shiina S.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Mungall A.J.,et al.Nature 425:805-811(2003).
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