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CAD Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P |
|---|---|
| Primary Accession | P27708 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 243kDa |
| Dilution | IHC-P (10 µg/ml), WB (1:500-1:3000), |
| Gene ID | 790 |
|---|---|
| Other Names | CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, 6.3.5.5, Aspartate carbamoyltransferase, 2.1.3.2, Dihydroorotase, 3.5.2.3, CAD |
| Target/Specificity | Human CAD. Predicted cross-reactivity based on amino acid sequence homology: mouse (92%), rat (92%). |
| Reconstitution & Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Precautions | CAD Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CAD (HGNC:1424) |
|---|---|
| Function | Multifunctional protein that encodes the first 3 enzymatic activities of the de novo pyrimidine pathway: carbamoylphosphate synthetase (CPSase; EC 6.3.5.5), aspartate transcarbamylase (ATCase; EC 2.1.3.2) and dihydroorotase (DHOase; EC 3.5.2.3). The CPSase-function is accomplished in 2 steps, by a glutamine-dependent amidotransferase activity (GATase) that binds and cleaves glutamine to produce ammonia, followed by an ammonium-dependent carbamoyl phosphate synthetase, which reacts with the ammonia, hydrogencarbonate and ATP to form carbamoyl phosphate. The endogenously produced carbamoyl phosphate is sequestered and channeled to the ATCase active site. ATCase then catalyzes the formation of carbamoyl-L-aspartate from L-aspartate and carbamoyl phosphate. In the last step, DHOase catalyzes the cyclization of carbamoyl aspartate to dihydroorotate. |
| Cellular Location | Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth |
| Volume | 100 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
References
Iwahana H.,et al.Biochem. Biophys. Res. Commun. 219:249-255(1996).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (FEB-2008) to UniProtKB.
Davidson J.N.,et al.DNA Cell Biol. 9:667-676(1990).
Zimmermann B.H.,et al.Biochemistry 34:7038-7046(1995).
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