|Application ||WB, IHC-P|
|Dilution||IHC-P (10 µg/ml), WB (1:500-1:3000),|
|Other Names||CAD protein, Glutamine-dependent carbamoyl-phosphate synthase, 126.96.36.199, Aspartate carbamoyltransferase, 188.8.131.52, Dihydroorotase, 184.108.40.206, CAD|
|Target/Specificity||Human CAD. Predicted cross-reactivity based on amino acid sequence homology: mouse (92%), rat (92%).|
|Reconstitution & Storage||Aliquot and store at -20°C. Minimize freezing and thawing.|
|Precautions||CAD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytosolic and unphosphorylated in resting cells, translocates to the nucleus in response to EGF stimulation, nuclear import promotes optimal cell growth|
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Provided below are standard protocols that you may find useful for product applications.
This protein is a "fusion" protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Iwahana H.,et al.Biochem. Biophys. Res. Commun. 219:249-255(1996).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Bienvenut W.V.,et al.Submitted (FEB-2008) to UniProtKB.
Davidson J.N.,et al.DNA Cell Biol. 9:667-676(1990).
Zimmermann B.H.,et al.Biochemistry 34:7038-7046(1995).
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