|Application ||WB, IHC-P, IF, ICC|
|Dilution||IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000)|
|Other Names||Melanoma inhibitory activity protein 3, C219-reactive peptide, D320, Transport and Golgi organization protein 1, MIA3, KIAA0268, TANGO, TANGO1|
|Target/Specificity||Human MIA3. Predicted cross-reactivity based on amino acid sequence homology: bovine (89%).|
|Reconstitution & Storage||Aliquot and store at -20°C. Minimize freezing and thawing.|
|Precautions||TANGO / MIA3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed:27138255).|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein Note=Localizes at endoplasmic reticulum exit sites. After loading of COL7A1 into transport carriers, it is not incorporated into COPII carriers and remains in the endoplasmic reticulum membrane|
|Tissue Location||Broadly expressed, except in bone marrow and peripheral blood mononuclear cells. Down-regulated in melanoma tissue.|
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Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Bosserhoff A.K.,et al.Gene Expr. Patterns 4:473-479(2004).
Nagase T.,et al.DNA Res. 3:321-329(1996).
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