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HIF2A / EPAS1 Antibody

Rabbit Polyclonal Antibody

     
  • IF - HIF2A / EPAS1 Antibody ALS13255
    Immunofluorescence of paraformaldehyde-fixed HeLa, using HIF2 alpha antibody at 1:200 dilution.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession Q99814
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 96kDa
Dilution IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000)
Additional Information
Gene ID 2034
Other Names Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, EPAS1, BHLHE73, HIF2A, MOP2, PASD2
Target/Specificity Mouse (97%), Chicken (94%), Pig (99%), Rat (95%), Xenopus laevis (86%), Bovine (98%), Xenopus tropicalis (86%)
Reconstitution & Storage Aliquot and store at -20°C. Minimize freezing and thawing.
PrecautionsHIF2A / EPAS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EPAS1
Synonyms BHLHE73, HIF2A, MOP2, PASD2
Function Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Cellular Location Nucleus {ECO:0000250|UniProtKB:P97481, ECO:0000255|PROSITE-ProRule:PRU00981}. Nucleus speckle {ECO:0000250|UniProtKB:P97481}. Note=Colocalizes with HIF3A in the nucleus and speckles. {ECO:0000250|UniProtKB:P97481}
Tissue Location Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells
Volume 50 µl
Research Areas
Citations (0)

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Background

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.

References

Tian H.,et al.Genes Dev. 11:72-82(1997).
Hogenesch J.B.,et al.J. Biol. Chem. 272:8581-8593(1997).
Ema M.,et al.EMBO J. 18:1905-1914(1999).
Furlow P.W.,et al.J. Biol. Chem. 284:9050-9058(2009).
Percy M.J.,et al.Blood 111:5400-5402(2008).

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$ 395.00
Cat# ALS13255
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