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BUB1B / BubR1 Antibody (clone 2G5)

Mouse Monoclonal Antibody

     
  • WB - BUB1B / BubR1 Antibody (clone 2G5) ALS13281
    BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
    detail
  • IHC - BUB1B / BubR1 Antibody (clone 2G5) ALS13281
    Anti-BUB1B / BubR1 antibody IHC of human skin.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E, PLA, RNAi
Primary Accession O60566
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G5
Calculated MW 120kDa
Dilution IHC-P (5 µg/ml)
Additional Information
Gene ID 701
Other Names Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, 2.7.11.1, MAD3/BUB1-related protein kinase, hBUBR1, Mitotic checkpoint kinase MAD3L, Protein SSK1, BUB1B, BUBR1, MAD3L, SSK1
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsBUB1B / BubR1 Antibody (clone 2G5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BUB1B
Synonyms BUBR1, MAD3L, SSK1
Function Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase- promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1
Tissue Location Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index
Research Areas
Citations (0)
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Background

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

References

Donadelli R.,et al.Biochem. Biophys. Res. Commun. 246:881-887(1998).
Taylor S.S.,et al.J. Cell Biol. 142:1-11(1998).
Chan G.K.T.,et al.J. Cell Biol. 143:49-63(1998).
Cahill D.P.,et al.Nature 392:300-303(1998).
Davenport J.W.,et al.Genomics 55:113-117(1999).

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Discontinued
Cat# ALS13281
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