|Application ||WB, IHC-P, E, RNAi|
|Dilution||IHC-P (5 µg/ml)|
|Other Names||Aspartate--tRNA ligase, cytoplasmic, 188.8.131.52, Aspartyl-tRNA synthetase, AspRS, Cell proliferation-inducing gene 40 protein, DARS|
|Reconstitution & Storage||Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||DARS Antibody (clone 2F11) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.|
|Cellular Location||Cytoplasm, cytosol|
|Tissue Location||Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporaal cortex and the frontal polar cortex. The cerebellum, cereral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon|
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Provided below are standard protocols that you may find useful for product applications.
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Jacobo-Molina A.,et al.J. Biol. Chem. 264:16608-16612(1989).
Kim J.W.,et al.Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
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