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DAZ1 / DAZ Antibody (clone 3G10)

Mouse Monoclonal Antibody

     
  • WB - DAZ1 / DAZ Antibody (clone 3G10) ALS13299
    DAZ1 monoclonal antibody, clone 3G10. Western blot of DAZ1 expression in human pancreas.
  • IF - DAZ1 / DAZ Antibody (clone 3G10) ALS13299
    Immunofluorescence of monoclonal antibody to DAZ1 on HeLa cell. [antibody concentration 10 ug/ml].
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9NQZ3
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3G10
Calculated MW 83kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 1617
Other Names Deleted in azoospermia protein 1, DAZ1, DAZ, SPGY
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsDAZ1 / DAZ Antibody (clone 3G10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DAZ1
Synonyms DAZ, SPGY
Function RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells.
Cellular Location Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis
Tissue Location Testis-specific. Expression restricted to premeiotic germ cells, particularly in spermatogonia (at protein level).
Research Areas
Citations (0)

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Background

RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells.

References

Skaletsky H.,et al.Nature 423:825-837(2003).
Saxena R.,et al.Genomics 67:256-267(2000).
Tsui S.,et al.Genomics 65:266-273(2000).
Reijo R.A.,et al.Biol. Reprod. 63:1490-1496(2000).
Ruggiu M.,et al.Gene 252:119-126(2000).

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$ 450.00
Cat# ALS13299
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