TSC22D3 / GILZ Antibody (clone 3A5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | Q99576 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 3A5 |
Calculated MW | 15kDa |
Dilution | IF (10 µg/ml), IHC-P (5 µg/ml), |
Gene ID | 1831 |
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Other Names | TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP, hDIP, Delta sleep-inducing peptide immunoreactor, Glucocorticoid-induced leucine zipper protein, GILZ, TSC-22-like protein, TSC-22-related protein, TSC-22R, TSC22D3, DSIPI, GILZ |
Reconstitution & Storage | Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles. |
Precautions | TSC22D3 / GILZ Antibody (clone 3A5) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TSC22D3 (HGNC:3051) |
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Function | Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11 (PubMed:15031210). In macrophages, plays a role in the anti- inflammatory and immunosuppressive effects of glucocorticoids and IL10 (PubMed:12393603). In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation and thereby NFKB1 DNA-binding activities (PubMed:11468175). In vitro, suppresses AP-1 transcription factor complex DNA-binding activities (By similarity). |
Cellular Location | [Isoform 1]: Cytoplasm {ECO:0000250|UniProtKB:Q9Z2S7}. Nucleus {ECO:0000250|UniProtKB:Q9Z2S7} Note=Localization depends on differentiation status of myoblasts (By similarity). In undifferentiated myoblasts; localizes to the cytoplasm, but in differentiating myoblast; localizes to the nucleus (By similarity). {ECO:0000250|UniProtKB:Q9Z2S7} |
Tissue Location | Ubiquitously expressed, including in the fetal brain and liver (PubMed:26752201). Expressed in brain, lung, spleen and skeletal muscle (PubMed:11313722, PubMed:12393603). Lower levels detected in heart and kidney (PubMed:11313722, PubMed:12393603). Not detected in the pancreas (PubMed:11313722). In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage (PubMed:12393603). Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes (PubMed:11313722). Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells (PubMed:12393603) |
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Background
Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 inhibits myogenic differentiation and mediates anti- myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG (By similarity).
References
Vogel P.,et al.Biochim. Biophys. Acta 1309:200-204(1996).
Cannarile L.,et al.Cell Death Differ. 8:201-203(2001).
Wistow G.J.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Okada T.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Kim M.K.,et al.Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
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