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>   home   >   Products   >   Primary Antibodies   >   ELAVL1 / HUR Antibody (clone 4G8)   

ELAVL1 / HUR Antibody (clone 4G8)

Mouse Monoclonal Antibody

     
  • WB - ELAVL1 / HUR Antibody (clone 4G8) ALS13304
    ELAVL1 monoclonal antibody clone 4G8 Western blot of ELAVL1 expression in HeLa.
    detail
  • IF - ELAVL1 / HUR Antibody (clone 4G8) ALS13304
    Immunofluorescence of monoclonal antibody to ELAVL1 on HeLa cell (antibody concentration 10 ug/ml).
    detail
  • IHC - ELAVL1 / HUR Antibody (clone 4G8) ALS13304
    Anti-HuR antibody IHC of human breast.
    detail
  • IHC - ELAVL1 / HUR Antibody (clone 4G8) ALS13304
    Anti-HuR antibody IHC of human kidney.
    detail
  • IHC - ELAVL1 / HUR Antibody (clone 4G8) ALS13304
    Anti-HuR antibody IHC of human testis.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q15717
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4G8
Calculated MW 36kDa
Dilution IF (10 µg/ml), IHC-P (10 µg/ml),
Additional Information
Gene ID 1994
Other Names ELAV-like protein 1, Hu-antigen R, HuR, ELAVL1, HUR
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsELAVL1 / HUR Antibody (clone 4G8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ELAVL1
Synonyms HUR
Function RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462, PubMed:31358969). Involved in embryonic stem cell (ESC) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESC differentiation (By similarity). Has also been shown to be capable of binding to m6A-containing mRNAs and contributes to MYC stability by binding to m6A-containing MYC mRNAs (PubMed:32245947). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412, PubMed:14731398). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR (PubMed:29180010).
Cellular Location Cytoplasm. Nucleus. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:P70372}. Cytoplasm, P-body. Note=Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462).Localizes to the stress granules in the presence of PLEKHN1 (By similarity). {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:18285462}
Tissue Location Ubiquitous. Detected in brain, liver, thymus and muscle.
Citations (0)
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Background

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation. Binds to poly-U elements and AU- rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA.

References

Ma W.-J.,et al.J. Biol. Chem. 271:8144-8151(1996).
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS13304
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