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>   home   >   Products   >   Primary Antibodies   >   MBNL1 / MBNL Antibody (clone 3E7)   

MBNL1 / MBNL Antibody (clone 3E7)

Mouse Monoclonal Antibody

     
  • WB - MBNL1 / MBNL Antibody (clone 3E7) ALS13356
    MBNL1 monoclonal antibody clone 3E7 Western blot of MBNL1 expression in HeLa NE.
  • IF - MBNL1 / MBNL Antibody (clone 3E7) ALS13356
    Immunofluorescence of monoclonal antibody to MBNL1 on HeLa cell (antibody concentration 10 ug/ml).
  • IHC - MBNL1 / MBNL Antibody (clone 3E7) ALS13356
    Anti-MBNL1 antibody IHC of human heart.
  • IHC - MBNL1 / MBNL Antibody (clone 3E7) ALS13356
    Anti-MBNL1 antibody IHC of human skeletal muscle.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9NR56
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E7
Calculated MW 42kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 4154
Other Names Muscleblind-like protein 1, Triplet-expansion RNA-binding protein, MBNL1, EXP, KIAA0428, MBNL
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsMBNL1 / MBNL Antibody (clone 3E7) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBNL1
Synonyms EXP, KIAA0428, MBNL
Function Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y- 3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.
Cellular Location Nucleus Cytoplasm. Cytoplasmic granule. Note=Localized with DDX1, TIAL1 and YBX1 in stress granules upon stress (PubMed:18335541). Localized in the cytoplasm of multinucleated myotubes (PubMed:18335541) Colocalizes with nuclear foci of retained expanded-repeat transcripts in myotubes from patients affected by myotonic dystrophy (PubMed:10970838, PubMed:11590133, PubMed:11929853)
Tissue Location Highly expressed in cardiac, skeletal muscle and during myoblast differentiation. Weakly expressed in other tissues (at protein level). Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Citations (0)

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Background

Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y- 3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues.

References

Borsani G.,et al.Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
Ishikawa K.,et al.DNA Res. 4:307-313(1997).
Miller J.W.,et al.EMBO J. 19:4439-4448(2000).
Mankodi A.,et al.Hum. Mol. Genet. 10:2165-2170(2001).
Pascual M.,et al.Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.

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$ 450.00
Cat# ALS13356
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