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SNRPA / U1A Antibody (clone 3F9-1F7)

Mouse Monoclonal Antibody

     
  • WB - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    SNRPA monoclonal antibody (M01), clone 3F9-1F7 Western blot of SNRPA expression in HeLa.
  • WB - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    Western blot of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody...
  • IF - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell (antibody concentration 10 ug/ml).
  • IHC - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    Anti-SNRPA antibody IHC of human small intestine, submucosal plexus.
  • IHC - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    Anti-SNRPA antibody IHC of human testis.
  • IHC - SNRPA / U1A Antibody (clone 3F9-1F7) ALS13397
    Anti-SNRPA antibody IHC of human tonsil.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P09012
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F9-1F7
Calculated MW 31kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 6626
Other Names U1 small nuclear ribonucleoprotein A, U1 snRNP A, U1-A, U1A, SNRPA
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsSNRPA / U1A Antibody (clone 3F9-1F7) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SNRPA
Function Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.
Cellular Location Nucleus.
Citations (0)

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Background

Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.

References

Nelissen R.L.H.,et al.Gene 102:189-196(1991).
Sillekens P.T.G.,et al.EMBO J. 6:3841-3848(1987).
Bienvenut W.V.,et al.Submitted (JAN-2004) to UniProtKB.
Lutz C.S.,et al.RNA 4:1493-1499(1998).
Dephoure N.,et al.Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).

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$ 450.00
Cat# ALS13397
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