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SOX9 Antibody (clone 3C10)

Mouse Monoclonal Antibody

     
  • WB - SOX9 Antibody (clone 3C10) ALS13398
    SOX9 monoclonal antibody clone 3C10 Western blot of SOX9 expression in HepG2.
    detail
  • IF - SOX9 Antibody (clone 3C10) ALS13398
    Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell (antibody concentration 10 ug/ml).
    detail
  • IHC - SOX9 Antibody (clone 3C10) ALS13398
    Anti-SOX9 antibody IHC of human colon, myenteric plexus.
    detail
  • IHC - SOX9 Antibody (clone 3C10) ALS13398
    Anti-SOX9 antibody IHC of human prostate.
    detail
  • IHC - SOX9 Antibody (clone 3C10) ALS13398
    Anti-SOX9 antibody IHC of human tonsil.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P48436
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3C10
Calculated MW 56kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 6662
Other Names Transcription factor SOX-9, SOX9
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsSOX9 Antibody (clone 3C10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX9 {ECO:0000303|PubMed:7990924, ECO:0000312|HGNC:HGNC:11204}
Function Transcription factor that plays a key role in chondrocytes differentiation and skeletal development (PubMed:24038782). Specifically binds the 5'-ACAAAG-3' DNA motif present in enhancers and super-enhancers and promotes expression of genes important for chondrogenesis, including cartilage matrix protein-coding genes COL2A1, COL4A2, COL9A1, COL11A2 and ACAN, SOX5 and SOX6 (PubMed:8640233). Also binds to some promoter regions (By similarity). Plays a central role in successive steps of chondrocyte differentiation (By similarity). Absolutely required for precartilaginous condensation, the first step in chondrogenesis during which skeletal progenitors differentiate into prechondrocytes (By similarity). Together with SOX5 and SOX6, required for overt chondrogenesis when condensed prechondrocytes differentiate into early stage chondrocytes, the second step in chondrogenesis (By similarity). Later, required to direct hypertrophic maturation and block osteoblast differentiation of growth plate chondrocytes: maintains chondrocyte columnar proliferation, delays prehypertrophy and then prevents osteoblastic differentiation of chondrocytes by lowering beta-catenin (CTNNB1) signaling and RUNX2 expression (By similarity). Also required for chondrocyte hypertrophy, both indirectly, by keeping the lineage fate of chondrocytes, and directly, by remaining present in upper hypertrophic cells and transactivating COL10A1 along with MEF2C (By similarity). Low lipid levels are the main nutritional determinant for chondrogenic commitment of skeletal progenitor cells: when lipids levels are low, FOXO (FOXO1 and FOXO3) transcription factors promote expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Mechanistically, helps, but is not required, to remove epigenetic signatures of transcriptional repression and deposit active promoter and enhancer marks at chondrocyte-specific genes (By similarity). Acts in cooperation with the Hedgehog pathway-dependent GLI (GLI1 and GLI3) transcription factors (By similarity). In addition to cartilage development, also acts as a regulator of proliferation and differentiation in epithelial stem/progenitor cells: involved in the lung epithelium during branching morphogenesis, by balancing proliferation and differentiation and regulating the extracellular matrix (By similarity). Controls epithelial branching during kidney development (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00267, ECO:0000269|PubMed:8640233}
Research Areas
Citations (0)
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Background

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

References

Foster J.W.,et al.Nature 372:525-530(1994).
Wagner T.,et al.Cell 79:1111-1120(1994).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Cox J.J.,et al.N. Engl. J. Med. 364:91-93(2011).

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Discontinued
Cat# ALS13398
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