|Application ||WB, IHC-P, IF, E|
|Dilution||IF (10 µg/ml), IHC-P (5 µg/ml),|
|Other Names||Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162|
|Reconstitution & Storage||Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||SF1 Antibody (clone 2E12) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.|
|Tissue Location||Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.|
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Provided below are standard protocols that you may find useful for product applications.
Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Arning S.,et al.RNA 2:794-810(1996).
Caslini C.,et al.Genomics 42:268-277(1997).
Toda T.,et al.Hum. Mol. Genet. 3:465-470(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).
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