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SF1 Antibody (clone 2E12)

Mouse Monoclonal Antibody

     
  • IF - SF1 Antibody (clone 2E12) ALS13439
    Immunofluorescence of monoclonal antibody to SF1 on HeLa cell (antibody concentration 10 ug/ml).
  • IHC - SF1 Antibody (clone 2E12) ALS13439
    Anti-SF1 antibody IHC of human adrenal.
  • IHC - SF1 Antibody (clone 2E12) ALS13439
    Anti-SF1 antibody IHC of human placenta.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q15637
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E12
Calculated MW 68kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 7536
Other Names Splicing factor 1, Mammalian branch point-binding protein, BBP, mBBP, Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF1, ZFM1, ZNF162
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsSF1 Antibody (clone 2E12) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SF1
Synonyms ZFM1, ZNF162
Function Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
Cellular Location Nucleus.
Tissue Location Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Citations (0)

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Background

Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.

References

Arning S.,et al.RNA 2:794-810(1996).
Caslini C.,et al.Genomics 42:268-277(1997).
Toda T.,et al.Hum. Mol. Genet. 3:465-470(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Taylor T.D.,et al.Nature 440:497-500(2006).

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$ 450.00
Cat# ALS13439
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