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G3BP1 / G3BP Antibody (clone 2F3)

Mouse Monoclonal Antibody

     
  • WB - G3BP1 / G3BP Antibody (clone 2F3) ALS13459
    Western blot of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody clone 2F3.
  • WB - G3BP1 / G3BP Antibody (clone 2F3) ALS13459
    G3BP monoclonal antibody clone 2F3 Western blot of G3BP expression in A-431.
  • IF - G3BP1 / G3BP Antibody (clone 2F3) ALS13459
    Immunofluorescence of monoclonal antibody to G3BP on HeLa cell (antibody concentration 10 ug/ml).
  • IHC - G3BP1 / G3BP Antibody (clone 2F3) ALS13459
    Anti-G3BP1 / G3BP antibody IHC of human breast.
  • IHC - G3BP1 / G3BP Antibody (clone 2F3) ALS13459
    Anti-G3BP1 / G3BP antibody IHC of human colon.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E, RNAi
Primary Accession Q13283
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F3
Calculated MW 52kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 10146
Other Names Ras GTPase-activating protein-binding protein 1, G3BP-1, 3.6.4.12, 3.6.4.13, ATP-dependent DNA helicase VIII, hDH VIII, GAP SH3 domain-binding protein 1, G3BP1, G3BP
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsG3BP1 / G3BP Antibody (clone 2F3) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name G3BP1
Synonyms G3BP
Function May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
Cellular Location Cytoplasm. Cytoplasm, cytosol. Cytoplasmic granule. Cell membrane. Nucleus. Note=Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
Tissue Location Ubiquitous.
Citations (0)

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Background

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium- dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

References

Parker F.,et al.Mol. Cell. Biol. 16:2561-2569(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.

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$ 450.00
Cat# ALS13459
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