|Application ||WB, IHC-P, IF, E|
|Dilution||IF (10 µg/ml), IHC-P (5 µg/ml),|
|Other Names||E3 ubiquitin-protein ligase Topors, 6.3.2.-, SUMO1-protein E3 ligase Topors, Topoisomerase I-binding RING finger protein, Topoisomerase I-binding arginine/serine-rich protein, Tumor suppressor p53-binding protein 3, p53-binding protein 3, p53BP3, TOPORS, LUN, TP53BPL|
|Reconstitution & Storage||Store at -20°C. Aliquot to avoid freeze/thaw cycles.|
|Precautions||TOPORS Antibody (clone 5G11) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage- induced cell death through IKBKE sumoylation.|
|Cellular Location||Nucleus. Nucleus, PML body. Note=Localizes to discrete nuclear foci which partly overlap with PML nuclear bodies. Targeted to PML nuclear bodies upon DNA damage|
|Tissue Location||Expressed at highest levels in testis and at lower levels in adrenal gland, bone marrow, brain, colon, heart, kidney, liver, muscle, ovary, pancreas, placenta, prostate, skeletal muscle, skin, small intestine, spleen, stomach, testis, thymus, thyroid and uterus. Expressed in the alveolar epithelium of the lung. Expression is commonly decreased in colon adenocarcinomas and lung cancers.|
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Functions as an E3 ubiquitin-protein ligase and as an E3 SUMO1-protein ligase. Probable tumor suppressor involved in cell growth, cell proliferation and apoptosis that regulates p53/TP53 stability through ubiquitin-dependent degradation. May regulate chromatin modification through sumoylation of several chromatin modification-associated proteins. May be involved in DNA damage- induced cell death through IKBKE sumoylation.
Haluska P. Jr.,et al.Nucleic Acids Res. 27:2538-2544(1999).
Chu D.,et al.J. Biol. Chem. 276:14004-14013(2001).
Humphray S.J.,et al.Nature 429:369-374(2004).
Zhou R.,et al.Gene 235:93-101(1999).
Rasheed Z.A.,et al.Exp. Cell Res. 277:152-160(2002).
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