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Gasdermin D / GSDMD Antibody (clone 3F12-1B2)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | P57764 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 3F12-1B2 |
| Calculated MW | 53kDa |
| Dilution | IF (10 µg/ml), IHC-P (5 µg/ml), |
| Gene ID | 79792 |
|---|---|
| Other Names | Gasdermin-D, Gasdermin domain-containing protein 1, GSDMD, DFNA5L, GSDMDC1 |
| Reconstitution & Storage | Store at -20°C. Aliquot to avoid freeze/thaw cycles. |
| Precautions | Gasdermin D / GSDMD Antibody (clone 3F12-1B2) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GSDMD {ECO:0000303|PubMed:26375003, ECO:0000312|HGNC:HGNC:25697} |
|---|---|
| Function | [Gasdermin-D]: Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals (PubMed:26375003, PubMed:26375259, PubMed:27281216). This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed:26375003, PubMed:26375259, PubMed:27281216). |
| Cellular Location | [Gasdermin-D]: Cytoplasm, cytosol. Inflammasome {ECO:0000250|UniProtKB:Q9D8T2}. Note=In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. {ECO:0000250|UniProtKB:Q9D8T2} [Gasdermin-D, N-terminal]: Cytoplasm, cytosol. Note=(Microbial infection) Upon infection by M.tuberculosis, localization to cell membrane is prevented by M.tuberculosis phosphatase PtpB that catalyzes dephosphorylation of phosphatidylinositol (4,5)-bisphosphate and phosphatidylinositol 4- phosphate, thereby inhibiting the membrane targeting of Gasdermin-D, N- terminal and subsequent cytokine release and pyroptosis [Gasdermin-D, C-terminal]: Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9D8T2} |
| Tissue Location | Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
References
Wang Y.-G.,et al.Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Katoh M.,et al.Int. J. Oncol. 25:765-770(2004).
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