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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Gasdermin D / GSDMD Antibody (clone 3F12-1B2)   

Gasdermin D / GSDMD Antibody (clone 3F12-1B2)

Mouse Monoclonal Antibody

     
  • WB - Gasdermin D / GSDMD Antibody (clone 3F12-1B2) ALS13498
    GSDMDC1 monoclonal antibody, clone 3F12-1B2 Western blot of GSDMDC1 expression in Jurkat.
  • IF - Gasdermin D / GSDMD Antibody (clone 3F12-1B2) ALS13498
    Immunofluorescence of monoclonal antibody to GSDMDC1 on HeLa cell (antibody concentration 10 ug/ml).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P57764
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F12-1B2
Calculated MW 53kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 79792
Other Names Gasdermin-D, Gasdermin domain-containing protein 1, GSDMD, DFNA5L, GSDMDC1
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsGasdermin D / GSDMD Antibody (clone 3F12-1B2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSDMD
Synonyms DFNA5L, GSDMDC1
Function Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)- bisphosphate, as well as phosphatidylinositol (3,4,5)- bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
Cellular Location Gasdermin-D: Cytoplasm, cytosol. Inflammasome {ECO:0000250|UniProtKB:Q9D8T2}. Note=In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor. {ECO:0000250|UniProtKB:Q9D8T2}
Tissue Location Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
Research Areas
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References

Wang Y.-G.,et al.Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Nusbaum C.,et al.Nature 439:331-335(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Katoh M.,et al.Int. J. Oncol. 25:765-770(2004).

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$ 450.00
Cat# ALS13498
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