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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   WDR77 / MEP50 Antibody (clone 3F10)   

WDR77 / MEP50 Antibody (clone 3F10)

Mouse Monoclonal Antibody

     
  • WB - WDR77 / MEP50 Antibody (clone 3F10) ALS13514
    WDR77 monoclonal antibody (M01), clone 3F10 Western blot of WDR77 expression in HeLa.
  • IHC - WDR77 / MEP50 Antibody (clone 3F10) ALS13514
    Anti-WDR77 antibody IHC of human skeletal muscle.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9BQA1
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F10
Calculated MW 37kDa
Dilution IHC-P (20 µg/ml)
Additional Information
Gene ID 79084
Other Names Methylosome protein 50, MEP-50, Androgen receptor cofactor p44, WD repeat-containing protein 77, p44/Mep50, WDR77, MEP50, WD45
Reconstitution & Storage Store at -20°C. Aliquot to avoid freeze/thaw cycles.
PrecautionsWDR77 / MEP50 Antibody (clone 3F10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR77
Synonyms MEP50, WD45
Function Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Cellular Location Nucleus. Cytoplasm. Note=Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors
Tissue Location Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Research Areas
Citations (0)

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Background

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

References

Friesen W.J.,et al.J. Biol. Chem. 277:8243-8247(2002).
Hosohata K.,et al.Mol. Cell. Biol. 23:7019-7029(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Yamada S.,et al.Oncogene 23:5901-5911(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).

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$ 450.00
Cat# ALS13514
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