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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CDC27 Antibody (C-Terminus)   

CDC27 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - CDC27 Antibody (C-Terminus) ALS13564
    Western blot of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 ug/ml.
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  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P30260
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 92kDa
Dilution IHC-P (5 µg/ml), WB (1-2 µg/ml),
Additional Information
Gene ID 996
Other Names Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, H-NUC, CDC27, ANAPC3, D0S1430E, D17S978E
Target/Specificity Human CDC27
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsCDC27 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC27
Synonyms ANAPC3, D0S1430E, D17S978E
Function Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, spindle
Research Areas
Citations (0)
citation

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Background

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

References

Tugendreich S.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:10031-10035(1993).
Chen P.L.,et al.Cell Growth Differ. 6:199-210(1995).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kraft C.,et al.EMBO J. 22:6598-6609(2003).

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$ 467.50
Cat# ALS13564
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