|Application ||WB, IHC-P, E|
|Dilution||IHC-P (10 µg/ml), WB (1:500-1:1000),|
|Other Names||F-box/WD repeat-containing protein 7, Archipelago homolog, hAgo, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, SEL-10, hCdc4, FBXW7 (HGNC:16712)|
|Target/Specificity||N terminus of the 69 kD isoform of the hCdc4 protein.|
|Reconstitution & Storage||+4°C or -20°C, Avoid repeated freezing and thawing.|
|Precautions||FBXW7 / FBW7 Antibody (N-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination (PubMed:17434132). Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1 (PubMed:11565034, PubMed:12354302, PubMed:11585921, PubMed:15103331, PubMed:14739463, PubMed:17558397, PubMed:17873522, PubMed:22608923). Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation (PubMed:14739463).|
|Cellular Location||Isoform 1: Nucleus, nucleoplasm Isoform 3: Nucleus, nucleolus|
|Tissue Location||Isoform 1 is widely expressed. Isoform 3 is expressed in brain.|
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Provided below are standard protocols that you may find useful for product applications.
Substrate recognition component of an SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1.
Winston J.T.,et al.Curr. Biol. 9:1180-1182(1999).
Moberg K.H.,et al.Nature 413:311-316(2001).
Strohmaier H.,et al.Nature 413:316-322(2001).
Li J.,et al.J. Neurochem. 82:1540-1548(2002).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
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