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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   BRCC45 / BRE Antibody   

BRCC45 / BRE Antibody

Rabbit Polyclonal Antibody

     
  • IF - BRCC45 / BRE Antibody ALS13645
    Immunofluorescence of paraformaldehyde-fixed HeLa, using BRE antibody at 1:200 dilution.
  • WB - BRCC45 / BRE Antibody ALS13645
    Sample(30 g of whole cell lysate).
  • WB - BRCC45 / BRE Antibody ALS13645
    Sample (30 ug of whole cell lysate).
  • IHC - BRCC45 / BRE Antibody ALS13645
    Anti-BRE antibody IHC of human brain, cortex.
  • IHC - BRCC45 / BRE Antibody ALS13645
    Anti-BRE antibody IHC of human breast.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession Q9NXR7
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 44kDa
Dilution IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000)
Additional Information
Gene ID 9577
Other Names BRCA1-A complex subunit BRE, BRCA1/BRCA2-containing complex subunit 45, Brain and reproductive organ-expressed protein, BRE (HGNC:1106), BRCC45
Target/Specificity Human and mouse BRE. Predicted cross-reactivity based on amino acid sequence homology: mouse (99%), rat (98%), bovine (99%), zebrafish (85%).
Reconstitution & Storage Aliquot and store at -20°C. Minimize freezing and thawing.
PrecautionsBRCC45 / BRE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BRE (HGNC:1106)
Synonyms BRCC45
Function Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX (PubMed:17525341, PubMed:19261746, PubMed:19261749, PubMed:19261748). In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer (PubMed:21282113, PubMed:19261748). Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:19214193, PubMed:24075985, PubMed:25283148, PubMed:26195665). Within the BRISC complex, acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity (PubMed:21282113). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). The BRISC complex plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985). Down- regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985). May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect (PubMed:15465831).
Cellular Location Cytoplasm. Nucleus. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs)
Tissue Location Expressed in all cell lines examined. Highly expressed in placenta.
Volume 50 µl
Research Areas
Citations (0)

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Background

Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti- apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.

References

Li L.,et al.Biochem. Biophys. Res. Commun. 206:764-774(1995).
Ching A.K.K.,et al.Biochem. Biophys. Res. Commun. 288:535-545(2001).
Dong Y.,et al.Mol. Cell 12:1087-1099(2003).
Keeton K.R.,et al.Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 395.00
Cat# ALS13645
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