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ELP3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, ICC |
|---|---|
| Primary Accession | Q9H9T3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 62kDa |
| Dilution | IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000) |
| Gene ID | 55140 |
|---|---|
| Other Names | Elongator complex protein 3, hELP3, 2.3.1.48, ELP3 |
| Target/Specificity | Human ELP3. Predicted cross-reactivity based on amino acid sequence homology: mouse (96%), bovine (98%), zebrafish (92%). |
| Reconstitution & Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Precautions | ELP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ELP3 {ECO:0000303|PubMed:15902492, ECO:0000312|HGNC:HGNC:20696} |
|---|---|
| Function | Catalytic tRNA acetyltransferase subunit of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5- methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29415125). In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity). May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed:29415125). Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337). Required for acetylation of GJA1 in the developing cerebral cortex (By similarity). |
| Cellular Location | Cytoplasm. Nucleus [Isoform 2]: Cytoplasm. Nucleus |
| Tissue Location | Expressed in the cerebellum and spinal motor neurons. |
| Volume | 50 µl |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Involved in acetylation of alpha-tubulin (PubMed:19185337). May also have a methyltransferase activity. Involved in cell migration. Involved in neurogenesis. Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha- tubulin acetylation (By similarity).
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Nusbaum C.,et al.Nature 439:331-335(2006).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Hawkes N.A.,et al.J. Biol. Chem. 277:3047-3052(2002).
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