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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SKA1 Antibody (C-Terminus)   

SKA1 Antibody (C-Terminus)

Rabbit Polyclonal Antibody

     
  • WB - SKA1 Antibody (C-Terminus) ALS13773
    Western blot of SKA1 in A549 cell lysate with SKA1 antibody at (A) 0.5 and (B) 1 ug/ml.
  • IHC - SKA1 Antibody (C-Terminus) ALS13773
    Anti-SKA1 antibody IHC of human brain, cerebellum.
  • IHC - SKA1 Antibody (C-Terminus) ALS13773
    Anti-SKA1 antibody IHC of human brain, cortex.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96BD8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29kDa
Dilution IHC-P (5 µg/ml), WB (0.5-1 µg/ml),
Additional Information
Gene ID 220134
Other Names Spindle and kinetochore-associated protein 1, SKA1, C18orf24
Target/Specificity Human SKA1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
PrecautionsSKA1 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SKA1
Synonyms C18orf24
Function Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation (PubMed:17093495, PubMed:19289083, PubMed:23085020). Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint (PubMed:17093495). The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies (PubMed:19289083). The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner (PubMed:19289083). Affinity for microtubules is synergistically enhanced in the presence of the ndc-80 complex and may allow the ndc-80 complex to track depolymerizing microtubules (PubMed:23085020). In the complex, it mediates the interaction with microtubules (PubMed:19289083, PubMed:23085020).
Cellular Location Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner (PubMed:17093495). Localizes to both the mitotic spindle and kinetochore-associated proteins (PubMed:17093495). Associates with kinetochores following microtubule attachment from prometaphase, through mid-anaphase and then vanishes in telophase (PubMed:17093495)
Research Areas
Citations (0)

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Background

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the interaction with microtubules.

References

Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Sauer G.,et al.Mol. Cell. Proteomics 4:35-43(2005).
Hanisch A.,et al.EMBO J. 25:5504-5515(2006).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).

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$ 395.00
Cat# ALS13773
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