|Dilution||IHC-P (5 µg/ml)|
|Other Names||Antigen KI-67, MKI67|
|Target/Specificity||middle of Ki67 protein|
|Reconstitution & Storage||Stable for 24 months when stored at 2-8°C.|
|Precautions||MKI67 / Ki67 Antibody (Internal) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).|
|Cellular Location||Chromosome. Nucleus. Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106)|
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Provided below are standard protocols that you may find useful for product applications.
Thought to be required for maintaining cell proliferation.
Schlueter C.,et al.J. Cell Biol. 123:513-522(1993).
Deloukas P.,et al.Nature 429:375-381(2004).
Gerdes J.,et al.Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
Sueishi M.,et al.J. Biol. Chem. 275:28888-28892(2000).
Takagi M.,et al.J. Biol. Chem. 276:25386-25391(2001).
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