|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||IHC-P (5 µg/ml), WB (1-2 µg/ml),|
|Other Names||Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, 22.214.171.124, Alkylated DNA repair protein alkB homolog 2, DNA oxidative demethylase ALKBH2, Oxy DC1, ALKBH2, ABH2|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.|
|Precautions||ABH2 / ALKBH2 Antibody (C-Terminus) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.|
|Cellular Location||Nucleus. Note=Detected in replication foci during S-phase|
|Tissue Location||Detected in colon, small intestine, ovary, testis, prostate, skeletal muscle, heart, liver and urinary bladder.|
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Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1- ethenoadenine (in vitro). Has strong preference for double- stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
Lin Y.,et al.Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Tsujikawa K.,et al.J. Cell. Mol. Med. 11:1105-1116(2007).
Scherer S.E.,et al.Nature 440:346-351(2006).
Duncan T.,et al.Proc. Natl. Acad. Sci. U.S.A. 99:16660-16665(2002).
Aas P.A.,et al.Nature 421:859-863(2003).
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