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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACTN4 Antibody (clone 4D10)   

ACTN4 Antibody (clone 4D10)

Mouse Monoclonal Antibody

     
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in PC-12.
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    ACTN4 monoclonal antibody (M01), clone 4D10 Western blot of ACTN4 expression in HeLa NE.
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in Raw 264.7.
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in MCF-7.
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    ACTN4 monoclonal antibody (M01), clone 4D10. Western blot of ACTN4 expression in NIH/3T3.
  • WB - ACTN4 Antibody (clone 4D10) ALS14018
    Western blot of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody...
  • IF - ACTN4 Antibody (clone 4D10) ALS14018
    Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20 ug/ml]
  • IHC - ACTN4 Antibody (clone 4D10) ALS14018
    Anti-ACTN4 / Alpha-Actinin-4 antibody IHC of human thyroid.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession O43707
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Clone Names 4D10
Calculated MW 105kDa
Dilution IF (20 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 81
Other Names Alpha-actinin-4, Non-muscle alpha-actinin 4, ACTN4 (HGNC:166)
Target/Specificity Human ACTN4
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsACTN4 Antibody (clone 4D10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTN4 (HGNC:166)
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).
Cellular Location Nucleus. Cytoplasm. Cell junction {ECO:0000250|UniProtKB:P57780}. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs Colocalizes with actin stress fibers. Nuclear translocation can be induced by the PI3 kinase inhibitor wortmannin or by cytochalasin D. Exclusively localized in the nucleus in a limited number of cell lines (breast cancer cell line MCF-7, oral floor cancer IMC- 2, and bladder cancer KU-7).
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Background

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (PubMed:15772161). Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (By similarity). May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (PubMed:22351778).

References

Nikolopoulos S.N.,et al.Oncogene 19:380-386(2000).
Aksenova V.I.U.,et al.Tsitologiia 54:25-32(2012).
Chakraborty S.,et al.Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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$ 450.00
Cat# ALS14018
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