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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   AML1 / RUNX1 Antibody (clone 2C10)   

AML1 / RUNX1 Antibody (clone 2C10)

Mouse Monoclonal Antibody

     
  • WB - AML1 / RUNX1 Antibody (clone 2C10) ALS14028
    RUNX1 monoclonal antibody (M06), clone 2C10 Western blot of RUNX1 expression in HeLa nuclear...
  • IF - AML1 / RUNX1 Antibody (clone 2C10) ALS14028
    Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml]
  • IHC - AML1 / RUNX1 Antibody (clone 2C10) ALS14028
    Anti-RUNX1 antibody IHC of human breast.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q01196
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2C10
Calculated MW 49kDa
Dilution IF (40 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 861
Other Names Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, RUNX1, AML1, CBFA2
Target/Specificity Human RUNX1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsAML1 / RUNX1 Antibody (clone 2C10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX1
Synonyms AML1, CBFA2
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532).
Cellular Location Nucleus.
Tissue Location Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood
Research Areas
Citations (0)

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Background

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation.

References

Ahn M.-Y.,et al.Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
Miyoshi H.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).
Sacchi N.,et al.Genes Chromosomes Cancer 11:226-236(1994).
Nucifora G.,et al.Blood 81:2728-2734(1993).
Levanon D.,et al.Genomics 23:425-432(1994).

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$ 450.00
Cat# ALS14028
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