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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   LIMK1 / LIMK Antibody (clone 2E9)   

LIMK1 / LIMK Antibody (clone 2E9)

Mouse Monoclonal Antibody

     
  • WB - LIMK1 / LIMK Antibody (clone 2E9) ALS14068
    LIMK1 monoclonal antibody (M05), clone 2E9 Western blot of LIMK1 expression in HeLa.
  • IHC - LIMK1 / LIMK Antibody (clone 2E9) ALS14068
    Anti-LIMK1 antibody IHC of human small intestine.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P53667
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E9
Calculated MW 73kDa
Dilution IHC-P (5 µg/ml)
Additional Information
Gene ID 3984
Other Names LIM domain kinase 1, LIMK-1, 2.7.11.1, LIMK1, LIMK
Target/Specificity Human LIMK1
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsLIMK1 / LIMK Antibody (clone 2E9) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIMK1
Synonyms LIMK
Function Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin- 2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).
Cellular Location Cytoplasm. Nucleus. Note=Predominantly found in the cytoplasm
Tissue Location Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle
Research Areas
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Background

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin- 2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).

References

Mizuno K.,et al.Oncogene 9:1605-1612(1994).
Frangiskakis J.M.,et al.Cell 86:59-69(1996).
Osborne L.R.,et al.Genomics 36:328-336(1996).
Edwards D.C.,et al.J. Biol. Chem. 274:11352-11361(1999).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 450.00
Cat# ALS14068
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