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>   home   >   Products   >   Primary Antibodies   >   POLK Antibody (clone 6F2)   

POLK Antibody (clone 6F2)

Mouse Monoclonal Antibody

     
  • WB - POLK Antibody (clone 6F2) ALS14095
    Western blot of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.
  • WB - POLK Antibody (clone 6F2) ALS14095
    Western blot of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone...
  • IF - POLK Antibody (clone 6F2) ALS14095
    Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
  • IHC - POLK Antibody (clone 6F2) ALS14095
    Anti-POLK antibody IHC of human spleen.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9UBT6
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6F2
Calculated MW 99kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 51426
Other Names DNA polymerase kappa, 2.7.7.7, DINB protein, DINP, POLK, DINB1
Target/Specificity Human POLK
Reconstitution & Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsPOLK Antibody (clone 6F2) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLK
Synonyms DINB1
Function DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
Cellular Location Nucleus. Note=Detected throughout the nucleus and at replication foci
Tissue Location Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
Citations (0)

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Background

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

References

Ogi T.,et al.Genes Cells 4:607-618(1999).
Gerlach V.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:11922-11927(1999).
Guo C.,et al.DNA Repair 4:397-402(2005).
Poltoratsky V.P.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Revert-Ros F.,et al.Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases.

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$ 450.00
Cat# ALS14095
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