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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RARA / RAR Alpha Antibody (clone 2C9-1F8)   

RARA / RAR Alpha Antibody (clone 2C9-1F8)

Mouse Monoclonal Antibody

     
  • WB - RARA / RAR Alpha Antibody (clone 2C9-1F8) ALS14145
    RARA monoclonal antibody clone 2C9-1F8 Western blot of RARA expression in A-431.
  • IF - RARA / RAR Alpha Antibody (clone 2C9-1F8) ALS14145
    Immunofluorescence of monoclonal antibody to RARA on A-431 cell. [antibody concentration 10 ug/ml]
  • IHC - RARA / RAR Alpha Antibody (clone 2C9-1F8) ALS14145
    Anti-RARA antibody IHC of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P10276
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2C9-1F8
Calculated MW 51kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml),
Additional Information
Gene ID 5914
Other Names Retinoic acid receptor alpha, RAR-alpha, Nuclear receptor subfamily 1 group B member 1, RARA, NR1B1
Target/Specificity Human RARA
Reconstitution & Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsRARA / RAR Alpha Antibody (clone 2C9-1F8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RARA
Synonyms NR1B1
Function Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.
Cellular Location Nucleus. Cytoplasm. Note=Nuclear localization depends on ligand binding, phosphorylation and sumoylation. Transloaction to the nucleus in the absence of ligand is dependent on activation of PKC and the downstream MAPK phosphorylation
Research Areas
Citations (0)

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Background

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand- dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

References

Giguere V.,et al.Nature 330:624-629(1987).
Chen Z.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:1178-1182(1994).
Hjalt T.A.H.,et al.Mamm. Genome 10:528-529(1999).
Parrado A.,et al.Nucleic Acids Res. 29:4901-4908(2001).
Zody M.C.,et al.Nature 440:1045-1049(2006).

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$ 450.00
Cat# ALS14145
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