|Application ||WB, IHC-P, IHC-Fr|
|Other Names||Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, ER-37, CHGA|
|Target/Specificity||The antibody is specific for chromogranin A. Specificity was ascertained by immunoblotting and immunohistochemistry. The antibody predominantly reacts with a 68 kD protein. Antigen was isolated from human Pheochromocytoma. Antigen location, cytoplasm ...|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||CHGA / Chromogranin A Antibody (clone LK2H10) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas. Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.|
|Cellular Location||Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle membrane. Secreted. Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions.|
|Tissue Location||GE-25 is found in the brain.|
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Provided below are standard protocols that you may find useful for product applications.
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas.
Konecki D.S.,et al.J. Biol. Chem. 262:17026-17030(1987).
Helman L.J.,et al.J. Biol. Chem. 263:11559-11563(1988).
Mouland A.J.,et al.J. Biol. Chem. 269:6918-6926(1994).
Mouland A.J.,et al.Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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