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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   PECAM-1 / CD31 Antibody (aa686-735)   

PECAM-1 / CD31 Antibody (aa686-735)

Rabbit Polyclonal Antibody

     
  • IF - PECAM-1 / CD31 Antibody (aa686-735) ALS14187
    Immunofluorescence of HeLa cells, using PECAM-1 Antibody.
  • WB - PECAM-1 / CD31 Antibody (aa686-735) ALS14187
    Western blot of extracts from Jurkat cells, using PECAM-1 Antibody.
  • IHC - PECAM-1 / CD31 Antibody (aa686-735) ALS14187
    Anti-CD31 antibody IHC of human tonsil, vessel.
  • IHC - PECAM-1 / CD31 Antibody (aa686-735) ALS14187
    Anti-CD31 antibody IHC of human tonsil.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P16284
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 83kDa
Dilution ELISA (1:20000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 5175
Other Names Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Target/Specificity PECAM-1 Antibody detects endogenous levels of total PECAM-1 protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsPECAM-1 / CD31 Antibody (aa686-735) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PECAM1
Function Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
Cellular Location Isoform Long: Cell membrane; Single-pass type I membrane protein Membrane raft. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells
Tissue Location Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level).
Research Areas
Citations (0)

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Background

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).

References

Simmons D.L.,et al.J. Exp. Med. 171:2147-2152(1990).
Stockinger H.,et al.J. Immunol. 145:3889-3897(1990).
Newman P.J.,et al.Science 247:1219-1222(1990).
Albelda S.M.,et al.J. Cell Biol. 114:1059-1068(1991).
Kirschbaum N.E.,et al.Blood 84:4028-4037(1994).

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$ 395.00
Cat# ALS14187
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