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>   home   >   Products   >   Primary Antibodies   >   DNMT3B Antibody (aa1-50)   

DNMT3B Antibody (aa1-50)

Rabbit Polyclonal Antibody

     
  • IF - DNMT3B Antibody (aa1-50) ALS14205
    Immunofluorescence of HeLa cells, using DNMT3B Antibody.
  • WB - DNMT3B Antibody (aa1-50) ALS14205
    Western blot of extracts from HT-29 cells, using DNMT3B Antibody.
  • IHC - DNMT3B Antibody (aa1-50) ALS14205
    Anti-DNMT3B antibody IHC of human testis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q9UBC3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 96kDa
Dilution ELISA (1:20000), IF (1:100-1:500), IHC-P (10 µg/ml), WB (1:500-1:1000) ,
Additional Information
Gene ID 1789
Other Names DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, 2.1.1.37, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, M.HsaIIIB, DNMT3B
Target/Specificity DNMT3B Antibody detects endogenous levels of total DNMT3B protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsDNMT3B Antibody (aa1-50) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT3B
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.
Cellular Location Nucleus
Tissue Location Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate
Volume 50 µl
Citations (0)

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Background

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.

References

Xie S.,et al.Gene 236:87-95(1999).
Xu G.-L.,et al.Nature 402:187-191(1999).
Ni J.,et al.Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).

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$ 395.00
Cat# ALS14205
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