|Application ||WB, IHC-P|
|Reactivity||Human, Rabbit, Monkey|
|Dilution||IHC-P (5 µg/ml)|
|Other Names||Nitric oxide synthase, endothelial, 22.214.171.124, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3|
|Target/Specificity||Recognizes eNOS/NOS III, Mr ~135kD. Species cross-reactivity: Human and bovine. Predicted to cross-react with mouse, guinea pig, porcine, rat and canine based on sequence homology.|
|Reconstitution & Storage||May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C|
|Precautions||NOS3 / eNOS Antibody (aa1181-1194) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.|
|Cellular Location||Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity|
|Tissue Location||Platelets, placenta, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Janssens S.P.,et al.J. Biol. Chem. 267:14519-14522(1992).
Janssens S.P.,et al.J. Biol. Chem. 267:22694-22694(1992).
Marsden P.A.,et al.FEBS Lett. 307:287-293(1992).
Marsden P.A.,et al.J. Biol. Chem. 268:17478-17488(1993).
Nadaud S.A.,et al.Biochem. Biophys. Res. Commun. 198:1027-1033(1994).
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