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>   home   >   Products   >   Primary Antibodies   >   EIF3D Antibody (aa101-150)   

EIF3D Antibody (aa101-150)

Rabbit Polyclonal Antibody

     
  • WB - EIF3D Antibody (aa101-150) ALS14212
    Western blot of extracts from NIH-3T3 cells, using EIF3D Antibody.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O15371
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 64kDa
Dilution ELISA (1:40000), IHC-P (10 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 8664
Other Names Eukaryotic translation initiation factor 3 subunit D {ECO:0000255|HAMAP-Rule:MF_03003}, eIF3d {ECO:0000255|HAMAP-Rule:MF_03003}, Eukaryotic translation initiation factor 3 subunit 7 {ECO:0000255|HAMAP-Rule:MF_03003}, eIF-3-zeta {ECO:0000255|HAMAP-Rule:MF_03003}, eIF3 p66, EIF3D {ECO:0000255|HAMAP-Rule:MF_03003}
Target/Specificity EIF3D Antibody detects endogenous levels of total EIF3D protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsEIF3D Antibody (aa101-150) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3D {ECO:0000255|HAMAP-Rule:MF_03003}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03003}.
Volume 50 µl
Citations (0)

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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

References

Asano K.,et al.J. Biol. Chem. 272:27042-27052(1997).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Dunham I.,et al.Nature 402:489-495(1999).

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$ 395.00
Cat# ALS14212
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