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NCF1 / p47phox / p47 phox Antibody (aa341-390)

Rabbit Polyclonal Antibody

     
  • WB - NCF1 / p47phox / p47 phox Antibody (aa341-390) ALS14220
    Western blot of extracts from HT-29/COLO205/HepG2 cells,, using p47 phox Antibody.
    detail
  • IHC - NCF1 / p47phox / p47 phox Antibody (aa341-390) ALS14220
    Anti-p47phox antibody IHC of human spleen.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P14598
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 45kDa
Dilution ELISA (1:5000), IHC-P (5 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 653361
Other Names Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A
Target/Specificity p47 phox Antibody detects endogenous levels of total p47 phox protein.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsNCF1 / p47phox / p47 phox Antibody (aa341-390) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCF1
Synonyms NOXO2, SH3PXD1A
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Detected in peripheral blood monocytes and neutrophils (at protein level).
Research Areas
Citations (0)
citation

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Background

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

References

Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).

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Discontinued
Cat# ALS14220
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