|Application ||WB, IHC-P, E|
|Reactivity||Human, Mouse, Rat|
|Dilution||ELISA (1:5000), IHC-P (5 µg/ml), WB (1:500-1:1000)|
|Other Names||Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3 and PX domain-containing protein 1A, p47-phox, NCF1, NOXO2, SH3PXD1A|
|Target/Specificity||p47 phox Antibody detects endogenous levels of total p47 phox protein.|
|Reconstitution & Storage||Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.|
|Precautions||NCF1 / p47phox / p47 phox Antibody (aa341-390) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).|
|Cellular Location||Cytoplasm, cytosol. Membrane; Peripheral membrane protein; Cytoplasmic side|
|Tissue Location||Detected in peripheral blood monocytes and neutrophils (at protein level).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989).
Volpp B.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:9563-9563(1989).
Lomax K.J.,et al.Science 245:409-412(1989).
Rodaway A.R.F.,et al.Mol. Cell. Biol. 10:5388-5396(1990).
Gorlach A.,et al.J. Clin. Invest. 100:1907-1918(1997).
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