PDE9A Antibody (clone 1E1)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | O76083 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 1E1 |
Calculated MW | 68kDa |
Dilution | IHC-P (5 µg/ml) |
Gene ID | 5152 |
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Other Names | High affinity cGMP-specific 3', 5'-cyclic phosphodiesterase 9A, 3.1.4.35, PDE9A |
Target/Specificity | Human PDE9A |
Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
Precautions | PDE9A Antibody (clone 1E1) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PDE9A (HGNC:8795) |
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Function | Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP (PubMed:9624146, PubMed:18757755, PubMed:21483814). Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart (PubMed:25799991). Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity). |
Cellular Location | [Isoform PDE9A1]: Cell projection, ruffle membrane. Cytoplasm, perinuclear region. Golgi apparatus. Endoplasmic reticulum. Cell membrane, sarcolemma [Isoform PDE9A3]: Cytoplasm. Endoplasmic reticulum |
Tissue Location | Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood (PubMed:9624146). Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate (PubMed:12565835). In brain, present in the cortex, cerebellum, and subiculum (at protein level) (PubMed:22328573). In heart, primarily localizes to myocytes (PubMed:25799991). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
References
Fisher D.A.,et al.J. Biol. Chem. 273:15559-15564(1998).
Guipponi M.,et al.Hum. Genet. 103:386-392(1998).
Rentero C.,et al.Biochem. Biophys. Res. Commun. 301:686-692(2003).
Wang P.,et al.Gene 314:15-27(2003).
Rentero C.,et al.BMC Mol. Biol. 7:39-39(2006).
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