|Application ||IHC-P, IF, E|
|Dilution||ELISA (1:40000), IF (1:100-1:500), IHC-P (10 µg/ml)|
|Other Names||MAP/microtubule affinity-regulating kinase 4, 22.214.171.124, MAP/microtubule affinity-regulating kinase-like 1, MARK4, KIAA1860, MARKL1|
|Target/Specificity||MARK4 Antibody detects endogenous levels of total MARK4 protein.|
|Reconstitution & Storage||Store at -20°C.|
|Precautions||MARK4 Antibody (aa461-510) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase (PubMed:15009667, PubMed:14594945, PubMed:23666762, PubMed:23184942). Phosphorylates the microtubule-associated protein MAPT (PubMed:14594945, PubMed:23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed:14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed:14594945, PubMed:25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed:23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed:23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed:25123532). Reduces neuronal cell survival (PubMed:15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed:23184942).|
|Cellular Location||Cytoplasm, cytoskeleton, microtubule organizing center, centrosome Cytoplasm, cytoskeleton, microtubule organizing center. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm Note=Localized at the tips of neurite-like processes in differentiated neuroblast cells. Detected in the cytoplasm and neuropil of the hippocampus.|
|Tissue Location||Ubiquitous. Isoform 2 is brain-specific (PubMed:11326310). Expressed at highest levels in brain and testis. Also expressed in heart, lung, liver, muscle, kidney and spleen (PubMed:14594945).|
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Provided below are standard protocols that you may find useful for product applications.
Drewes G.,et al.Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases.
Kato T.,et al.Neoplasia 3:4-9(2001).
Beghini A.,et al.Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 8:85-95(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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