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ASAH1 / Acid Ceramidase Antibody (clone 1A7)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q13510 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1A7 |
| Calculated MW | 45kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 427 |
|---|---|
| Other Names | Acid ceramidase, AC, ACDase, Acid CDase, 3.5.1.23, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, Putative 32 kDa heart protein, PHP32, Acid ceramidase subunit alpha, Acid ceramidase subunit beta, ASAH1, ASAH |
| Target/Specificity | Human ASAH1 |
| Reconstitution & Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | ASAH1 / Acid Ceramidase Antibody (clone 1A7) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ASAH1 (HGNC:735) |
|---|---|
| Synonyms | ASAH |
| Function | Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed:10610716, PubMed:7744740, PubMed:15655246, PubMed:11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed:10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed:7744740, PubMed:15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed:12764132, PubMed:12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro- phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed:12764132). Has also an N- acylethanolamine hydrolase activity (PubMed:15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed:17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed:22261821). |
| Cellular Location | Lysosome. Secreted. Note=Secretion is extremely low and localization to lysosomes is mannose-6-phosphate receptor-dependent |
| Tissue Location | Broadly expressed with higher expression in heart. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid.
References
Koch J.,et al.J. Biol. Chem. 271:33110-33115(1996).
Churchill J.R.,et al.Mol. Biol. Cell 6:418-418(1995).
Wieland S.J.,et al.Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases.
Fan M.M.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Zhang Z.,et al.Mol. Genet. Metab. 70:301-309(2000).
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