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AKR1B1 / Aldose Reductase Antibody (clone 2D12)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P15121 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2D12 |
| Calculated MW | 36kDa |
| Dilution | IHC-P (2.5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 231 |
|---|---|
| Other Names | Aldose reductase, AR, 1.1.1.21, Aldehyde reductase, Aldo-keto reductase family 1 member B1, AKR1B1, ALDR1 |
| Target/Specificity | Human AKR1B1 |
| Reconstitution & Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | AKR1B1 / Aldose Reductase Antibody (clone 2D12) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | AKR1B1 |
|---|---|
| Synonyms | ALDR1, ALR2 {ECO:0000303|PubMed:17368668 |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis- retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2- (5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS- carbonyls) (PubMed:21329684). |
| Cellular Location | Cytoplasm. |
| Tissue Location | Highly expressed in embryonic epithelial cells (EUE) in response to osmotic stress. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.
References
Bohren K.M.,et al.J. Biol. Chem. 264:9547-9551(1989).
Chung S.,et al.J. Biol. Chem. 264:14775-14777(1989).
Graham A.,et al.Nucleic Acids Res. 17:8368-8368(1989).
Grundmann U.,et al.DNA Cell Biol. 9:149-157(1990).
Nishimura C.,et al.J. Biol. Chem. 265:9788-9792(1990).
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