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DLD / Diaphorase / E3 Antibody (clone 1G11)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P09622 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 1G11 |
| Calculated MW | 54kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 1738 |
|---|---|
| Other Names | Dihydrolipoyl dehydrogenase, mitochondrial, 1.8.1.4, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein, DLD, GCSL, LAD, PHE3 |
| Target/Specificity | Human DLD |
| Reconstitution & Storage | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
| Precautions | DLD / Diaphorase / E3 Antibody (clone 1G11) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DLD |
|---|---|
| Synonyms | GCSL, LAD, PHE3 |
| Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched- chain amino acid-dehydrogenase complex) (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:17404228, PubMed:20160912, PubMed:20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2- oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity). |
| Cellular Location | Mitochondrion matrix. Nucleus. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q811C4}. Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2- oxoglutarate dehydrogenase complex is required for histone succinylation. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
References
Otulakowski G.,et al.J. Biol. Chem. 262:17313-17318(1987).
Pons G.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1422-1426(1988).
Feigenbaum A.S.,et al.Genomics 17:376-381(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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