DLD / Diaphorase / E3 Antibody (clone 2D4)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, E |
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Primary Accession | P09622 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 2D4 |
Calculated MW | 54kDa |
Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
Gene ID | 1738 |
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Other Names | Dihydrolipoyl dehydrogenase, mitochondrial, 1.8.1.4, Dihydrolipoamide dehydrogenase, Glycine cleavage system L protein, DLD, GCSL, LAD, PHE3 |
Target/Specificity | Human DLD |
Reconstitution & Storage | Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles. |
Precautions | DLD / Diaphorase / E3 Antibody (clone 2D4) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DLD |
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Synonyms | GCSL, LAD, PHE3 |
Function | Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of three alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched- chain amino acid-dehydrogenase complex) (PubMed:15712224, PubMed:16442803, PubMed:16770810, PubMed:17404228, PubMed:20160912, PubMed:20385101). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2- oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711). In monomeric form may have additional moonlighting function as serine protease (PubMed:17404228). Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity). |
Cellular Location | Mitochondrion matrix. Nucleus. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q811C4}. Cytoplasmic vesicle, secretory vesicle, acrosome. Note=Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2- oxoglutarate dehydrogenase complex is required for histone succinylation. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
References
Otulakowski G.,et al.J. Biol. Chem. 262:17313-17318(1987).
Pons G.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1422-1426(1988).
Feigenbaum A.S.,et al.Genomics 17:376-381(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Totoki Y.,et al.Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
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