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>   home   >   Products   >   Primary Antibodies   >   NME1 Antibody (clone 1D7)   

NME1 Antibody (clone 1D7)

Mouse Monoclonal Antibody

     
  • WB - NME1 Antibody (clone 1D7) ALS14391
    NME1 monoclonal antibody ALS14391. Western blot of NME1 expression in HeLa.
  • WB - NME1 Antibody (clone 1D7) ALS14391
    Western blot of NME1 expression in transfected 293T cell line by NME1 monoclonal antibody ALS14391
  • IF - NME1 Antibody (clone 1D7) ALS14391
    Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml]
  • IHC - NME1 Antibody (clone 1D7) ALS14391
    Anti-NME1 antibody IHC of human placenta.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E, IP
Primary Accession P15531
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1D7
Calculated MW 17kDa
Dilution IF (10 µg/ml), IHC-P (5 µg/ml), WB (1:500-1:1000)
Additional Information
Gene ID 4830
Other Names Nucleoside diphosphate kinase A, NDK A, NDP kinase A, 2.7.4.6, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23
Target/Specificity Human NME1
Reconstitution & Storage Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNME1 Antibody (clone 1D7) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NME1
Synonyms NDPKA, NM23
Function Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.
Cellular Location Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA
Tissue Location Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation
Citations (0)

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Background

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

References

Rosengard A.M.,et al.Nature 342:177-180(1989).
Gilles A.-M.,et al.J. Biol. Chem. 266:8784-8789(1991).
Wang L.,et al.Cancer Res. 53:717-720(1993).
Dooley S.,et al.Hum. Genet. 93:63-66(1994).
Ni X.,et al.J. Hum. Genet. 48:96-100(2003).

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$ 450.00
Cat# ALS14391
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