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KPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5)

Mouse Monoclonal Antibody

     
  • WB - KPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5) ALS14392
    Western blot of KPNA1 expression in HeLa cell lysate.
    detail
  • WB - KPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5) ALS14392
    Western blot of KPNA1 expression in transfected 293T cell line by KPNA1 monoclonal antibody.
    detail
  • IHC - KPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5) ALS14392
    Anti-KPNA1 / SRP1 antibody IHC of human testis.
    detail
  • SPECIFICATION
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P52294
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2A4-1B5
Calculated MW 60kDa
Dilution IHC-P (5 µg/ml), WB (1:500-1:1000),
Additional Information
Gene ID 3836
Other Names Importin subunit alpha-5, Karyopherin subunit alpha-1, Nucleoprotein interactor 1, NPI-1, RAG cohort protein 2, SRP1-beta, Importin subunit alpha-5, N-terminally processed, KPNA1, RCH2
Target/Specificity Human Importin Alpha-1
Reconstitution & Storage Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
PrecautionsKPNA1 / Importin Alpha 5 Antibody (clone 2A4-1B5) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KPNA1
Synonyms RCH2
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed:7892216, PubMed:8692858, PubMed:27713473). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed:7892216, PubMed:8692858, PubMed:27713473). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:7892216, PubMed:27713473). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin (PubMed:7892216). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:7892216).
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed ubiquitously.
Research Areas
Citations (0)
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Background

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS.

References

O'Neill R.E.,et al.Virology 206:116-125(1995).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Muzny D.M.,et al.Nature 440:1194-1198(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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Discontinued
Cat# ALS14392
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