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MAPK15 / ERK7 Antibody

Rabbit Polyclonal Antibody

     
  • IF - MAPK15 / ERK7 Antibody ALS14472
    Immunofluorescence of methanol-fixed A431, using MAPK15 antibody at 1:200 dilution.
    detail
  • WB - MAPK15 / ERK7 Antibody ALS14472
    Sample. A: 30g of HeLa S3 whole cell lysate. 7.5% SDS PAGE. MAPK15 antibody diluted at 1:1000
    detail
  • IHC - MAPK15 / ERK7 Antibody ALS14472
    Anti-MAPK15 antibody IHC of human brain, cerebellum.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, ICC
Primary Accession Q8TD08
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 60kDa
Dilution ICC (1:100-1:200), IF (1:100-1:200), IHC-P (10 µg/ml), WB (1:500-1:3000) ,
Additional Information
Gene ID 225689
Other Names Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, 2.7.11.24, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8, MAPK15, ERK7, ERK8
Target/Specificity Human MAPK15.
Reconstitution & Storage Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
PrecautionsMAPK15 / ERK7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAPK15 (HGNC:24667)
Function Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (PubMed:22948227, PubMed:24618899, PubMed:29021280, PubMed:21847093, PubMed:20733054). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation (PubMed:22948227). Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling (PubMed:29021280). Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar- coated proteins (PubMed:24618899). Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion (PubMed:21847093). Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA (PubMed:20733054). Regulates DA transporter (DAT) activity and protein expression via activation of RhoA (PubMed:28842414). In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (PubMed:26595526). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (PubMed:11875070, PubMed:16484222, PubMed:20638370, PubMed:19166846). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (By similarity). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (PubMed:21190936).
Cellular Location Cytoplasm, cytoskeleton, cilium basal body. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole Cytoplasmic vesicle, autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle {ECO:0000250|UniProtKB:Q80Y86}. Note=Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936) Translocates to the nucleus upon activation (PubMed:20638370). At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity). {ECO:0000250|UniProtKB:Q80Y86, ECO:0000269|PubMed:20638370, ECO:0000269|PubMed:21190936}
Tissue Location Widely expressed with a maximal expression in lung and kidney.
Volume 50 µl
Research Areas
Citations (0)
citation

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Background

In vitro, phosphorylates MBP.

References

Abe M.K.,et al.J. Biol. Chem. 277:16733-16743(2002).
Iavarone C.,et al.J. Biol. Chem. 281:10567-10576(2006).
Saelzler M.P.,et al.J. Biol. Chem. 281:16821-16832(2006).
Oppermann F.S.,et al.Mol. Cell. Proteomics 8:1751-1764(2009).

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Cat# ALS14472
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