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ATP6V1B2 Antibody (clone 2A5)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P21281 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Names | 2A5 |
| Calculated MW | 57kDa |
| Dilution | IHC-P (5 µg/ml), WB (1:500-1:1000), |
| Gene ID | 526 |
|---|---|
| Other Names | V-type proton ATPase subunit B, brain isoform, V-ATPase subunit B 2, Endomembrane proton pump 58 kDa subunit, HO57, Vacuolar proton pump subunit B 2, ATP6V1B2, ATP6B2, VPP3 |
| Target/Specificity | Human ATP6V1B2 |
| Reconstitution & Storage | Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. |
| Precautions | ATP6V1B2 Antibody (clone 2A5) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ATP6V1B2 |
|---|---|
| Synonyms | ATP6B2, VPP3 |
| Function | Non-catalytic subunit of the V1 complex of vacuolar(H+)- ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons (PubMed:33065002). V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment (PubMed:32001091). In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load (By similarity). |
| Cellular Location | Apical cell membrane. Melanosome. Cytoplasm {ECO:0000250|UniProtKB:P62814}. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250|UniProtKB:P62815}; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250|UniProtKB:P62815}; Peripheral membrane protein. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV |
| Tissue Location | Kidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
References
Nelson R.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:3541-3545(1992).
van Hille B.,et al.Biochem. J. 303:191-198(1994).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Lee B.S.,et al.J. Biol. Chem. 270:7320-7329(1995).
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